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蛋白纯化琼脂糖
产品介绍
PureCube His Affinity Agarose (NTA)是针对组氨酸标签的亲和层析产品，组氨酸标签是应用广泛的亲和层析标签，这种标签具有小尺寸、低免疫原性的特点和在多种天然或变性条件下的广泛适用性，NTA相对于IDA具有更高的洗脱液纯度。
产品优势：
1）德国制造，品质与信誉的坚实保证；
2）结合载量可高达80mg/mL，目前市面上最高结合能力；
3）更优越的DTT和EDTA稳定性和耐受性；
4）凝胶的多孔性和均匀粒径，使蛋白与凝胶之间有很好地相互作用；
5）适合低压环境下的纯化过程（大规模纯化制备或预装柱均可使用）；
6）高性价比，为研发、生产降低成本；
7）适用于大肠杆菌和真核细胞的裂解液和上清液；
8）同时可装载Co, Cu, Zn和其它金属离子。
优势详解：
1. 收率高，纯度高
Fig. 1: Over 20% more yield obtained with PureCube Ni-NTA Agarose. SDS-PAGE of GFP expressed in E. coli and purified in gravity columns with PureCube Ni-NTA Agarose and Ni-NTA resin from Competitor Q. 80 mg/mL protein yield was obtained with PureCube Ni-NTA Agarose (E1–E4, Cube) compared to 65 and 48 mg/mL, respectively, with the widely used alternative resins G and Q (E1–E4, Competitor G / Competitor Q).
Cube Biotech | |
蛋白结合能力 | > 80mg/mL |
1. 优越的DTT和EDTA稳定性
Fig. 2: NTA is robust in the presence of reducing and chelating agents. GFP-His was purified on gravity columns containing PureCube Ni-NTA Agarose after exposing the resin for 1 h to 3 concentrations of DTT or EDTA. NTA exhibits a shallow decay rate in binding capacity.
PureCube Ni-NTA琼脂糖在DTT和EDTA存在下非常坚实，在稳定性测试中，将PureCube Ni-NTA琼脂糖置于DTT与EDTA浓度逐渐升高的环境中处理1小时。再用处理过的凝胶在重力层析柱中纯化大肠杆菌表达的GFP-His，凝胶的蛋白结合能力因存在DTT与EDTA而下降，但是衰减率平缓，即使在1.5 mM EDTA，凝胶依然具有其最大结合能力的54%。
Cube Biotech | |
DTT稳定性 | <10mM |
EDTA稳定性 | <1.5mM |
应用 | Specific binding and purification of 6x his-tagged proteins |
特异性 | Affinity to His-tagged proteins |
结合能力 | >80 mg/mL |
Bead Ligand | Ni-NTA |
粒径大小 | 40 μm |
螯合剂稳定性 | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
保存 | Delivered as a 50 % suspension |
所需试剂耗材 | Lysis Buffer Wash Buffer Elution Buffer Ice bath Refrigerated centrifuge for 50 mL tube (min 10,000 x g) 50 mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment |
订货信息：
31101 | PureCube Ni-NTA Agarose (1 ml) |
31103 | PureCube Ni-NTA Agarose (10 ml) |
31105 | PureCube Ni-NTA Agarose (50 ml) |
31110 | PureCube Ni-NTA Agarose (250 ml) |
31112 | PureCube Ni-NTA Agarose (500 ml) |
31115 | PureCube Ni-NTA Agarose (1000 ml) |
31120 | PureCube Ni-NTA Agarose (5000 ml) |
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成都正民德思生物科技有限公司致力于成为中国与世界生物技术产业的纽带，以务实的原则将欧洲高科技、高质量、高效能的产品引进中国。正民德思从2013年起至今，作为生物制药和体外诊断领域相关产品的供应商，秉承“为客户增值”的企业价值观提供优质的服务，通过对合作伙伴、客户及社会的契约责任的高度重视，已积累与中国、德国、西班牙、奥地利、美国、芬兰等众多知名生命科学企业、生物技术公司、制药公司、科研院校的合作经验，并获得合作伙伴和客户的高度认可并保持稳固的战略合作关系，互相支持、共同发展。
目前，成都正民德思生物科技有限公司作为德国蛋白纯化产品生产商/服务商Cube Biotech生物科技公司中国独家总代理、西班牙生物磁性分离设备制造商SEPMAG中国独家总代理、西班牙免疫诊断试剂研发服务商Pragmatic Diagnostics公司的中国独家战略合作方，在成都成立Cube Biotech、SEPMAG和Pragmatic Diagnostics的中国总部办事处，全权负责在中国地区的营销事务、销售及售后服务等所有工作。
成都正民德思生物科技有限公司
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